In November, the Duke of Sussex denied permission to wear a wreath at the cenotaph on his behalf. Prince Harry made a personal request to Buckingham Palace, but was denied by the courtiers on the grounds that he was no longer representing the monarchy.
The Duke and Duchess of Sussex resigned as senior members of the Royal Family earlier this year.
His Majesty took “all two seconds” to decide on the subject of the wreath in the internal matter.
According to Rebecca English, royal correspondent for the Daily Mail, Prince Harry is believed to have been “deeply saddened” by the Queen’s decision.
Meghan Markle and Prince Harry immigrated to the US in March with their baby son Archie.
In November, Sussex published pictures of himself paying homage to flowers at the Los Angeles National Cemetery.
Palace sources said Her Majesty rejected the Duke’s request and made the decision “in less than a minute”, according to the newspaper.
An insider said: “When it comes to her Majesty’s diary, it’s a memorial Sunday, it’s the most important date on her calendar, and nothing has been done without her knowledge.”
They added: “People were suggesting the Palace’s reaction to what Harry asked was small, but it was the Queen’s decision.
Read more: ‘No new negotiations!’ The Queen asked Meghan and Harry to snatch the title
Leaving royal duties, Sussex has signed deals with streaming giants Netflix and Spotify.
Their employment choices may affect reports of what kind of relationship they have with the firm in the future.
A source told the Daily Mail: “Coming back means we have to undo all the business connections they’ve made, and clearly Harry and Meghan don’t want to do that.
“Harry and Meghan are clear where they want to be, and good luck to them.
“But subsequent career choices have lost any chance of retaining even a semi-official royal role.”
Royal critics have urged the Queen to remove the royal title of Sussex.
Good Morning Britain host Pierce Morgan took to Twitter to blast the couple for their “shameful” use of royal titles.
He wrote: “I think being a member of the Ropi family, they are a bunch of small waste, exploiting their royal titles for no commercial gain other than duty.
“It’s shameless, shameful and the Queen should stop doing that by removing the title from them.”
Express.co.uk contacted Buckingham Palace for comment.